Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Table 2

Gene expression on day 4 EB differentiation in rescue TIAR siRNA-treated cells.

	TIAR RNAi	Expressional control	Expressional TIAR
Nestin	1.03 ± 0.032	1.0	1.62 ± 0.057
Pax6	0.97 ± 0.055	1.0	1.43 ± 0.060
Flk1	1.06 ± 0.036	1.0	1.43 ± 0.035
Brachyury	0.97 ± 0.065	1.0	1.53 ± 0.040
Sox17	0.99 ± 0.047	1.0	1.48 ± 0.051
Gata6	0.99 ± 0.042	1.0	1.58 ± 0.045

by one-way ANOVA. TIAR RNAi: TIAR RNAi E14 cells; expressional control: TIAR RNAi E14 cells infected with an empty control pCDNA3.1-Hygro vector; expressional TIAR: TIAR RNAi E14 cells infected with pCDNA3.1-Hygro vector containing RNAi-resistant TIAR transgene.