Oleanolic Acid Induces Differentiation of Neural Stem Cells to Neurons: An Involvement of Transcription Factor Nkx-2.5

Figure 3

Effects of OA on the differentiation of NSCs. Single NSCs were seeded at a density of 50 cells/μL in PDL-coated 96-well plate in differentiation medium for 48 h without OA (a, b) or with 10 nM OA (c, d). Cells were incubated with primary antibodies to TuJ1 and GFAP and the corresponding secondary antibodies and visualized with Alexa-conjugated 594 F(ab)′2 goat anti-rabbit antibody and 488 Alexa-conjugated goat anti mouse IgG (H + L). The ratio of TuJ1, GFAP-positive cells against DAPI-stained cells was calculated. OA significantly increased the TuJ1-positive cells and decreased the GFAP-positive cells (e). The same cells were performed using Western blotting. OA significantly increased TuJ1, Mash1, and GFAP protein expression (f). Scale bars: 100 μm. Results were expressed as mean ± SD from three independent experiments. ^* versus control.

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