Bone Marrow-Derived Multipotent Stromal Cells Promote Myocardial Fibrosis and Reverse Remodeling of the Left Ventricle

Figure 1

Flow cytometry and induced differentiation analyses of MSCs. (a) The cultures were positive for CD73, CD90, and CD105 but negative for CD34, CD45, CD19, and CD11b. (b) Appearance of intracellular lipid-rich vacuoles during MSCs adipogenic differentiation was confirmed by Sudan III staining at day 14. (c) Differentiation of MSCs to chondrogenic lineage was visualized by staining for mucopolysaccharides with alcian blue at day 28. (d) Differentiation of MSCs to osteogenic lineage was visualized by staining for calcifications with alizarin red at day 21. (e) Microscopic evidence for effective labeling of MSCs with PKH26 (the nuclei are additionally stained with DAPI). Scale bars, 50 μm.

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