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Material | Analysis | Outcomes | Reference |
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rGO-Chitosan | SEM, Alizarin Red staining, and immunofluorescence | The differentiation on rGO-chitosan substrate was higher than the ones obtained on the chitosan substrata and polystyrene regardless of the use of osteogenic induction media | [21] |
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rGO-PEDOT | Immunofluorescence staining, Alizarin Red S staining | The multifunctional rGO-PEDOT bioelectronic interface was used for manipulating attachment and orientation of MSC. The device acted as a drug releasing model under electrical modulation | [83] |
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GO | Immunofluorescence, microcomputed tomography, and Goldner trichrome | The osteogenetic differentiation of human BMMSCs on Ti/GO substrate was higher compared to Ti substrate | [84] |
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GONR, rGONR | Immunofluorescence staining and Alizarin Red staining | Graphene nanogrids increase the osteogenic differentiation of BMSC; the differentiation coincides with the patterns of the nanogrids | [45] |
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CVD | Immunofluorescence staining | The cells adhered and proliferated more on CVD-grown graphene than on SiO2 substrates | [46] |
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CVD, GO | Immunofluorescence staining and Alizarin Red staining | Graphene was capable of preconcentrating osteogenic differentiation factors. GO strongly enhances adipogenic differentiation | [33] |
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CVD | Cell viability assay, immunofluorescence staining, and Alizarin Red staining | CVD-grown graphene allowed the proliferation of MSC and increased the differentiation towards osteoblast | [47] |
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3DGp | Immunofluorescence staining and SEM | 3DGp maintains MSC viability and promotes osteogenic differentiation without the use of chemical inducers | [52] |
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CaS-G | MTT, SEM, and RT-PCR | Cell adhesion was enhanced by adding 1.5% of graphene to the material as compared to the calcium silicate alone | [72] |
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SGH | MTT, H & E, immunofluorescence staining, and Alizarin staining | The self-supporting graphene hydrogel (SGH) film allows cell adhesion and proliferation and accelerates the osteogenic differentiation without chemical inducer | [66] |
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GO-CaP | Alizarin Red S staining RT PCR and immunofluorescence | The GO-CaP nanocomposite exhibited superior osteoinductivity compared to individual or combined effects of GO and CaP | [73] |
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Carbon nanotubes and graphene | SEM, Elisa, and H & E staining | Cells in PLLA composite scaffolds containing 3% wt of graphene presented higher expression of osteogenesis-related proteins, calcium deposition, and the formation of type I collagen | [80] |
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Graphene hydrogel | MTT and SEM | Graphene 3D hydrogel allows cell proliferation and attachment confirming the biocompatibility of the graphene hydrogel scaffolds | [82] |
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