TY - JOUR A2 - Mezey, Eva AU - Laranjeira, Paula AU - Gomes, Joana AU - Pedreiro, Susana AU - Pedrosa, Monia AU - Martinho, Antonio AU - Antunes, Brigida AU - Ribeiro, Tania AU - Santos, Francisco AU - Domingues, Rosario AU - Abecasis, Manuel AU - Trindade, Helder AU - Paiva, Artur PY - 2015 DA - 2015/04/28 TI - Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells SP - 819084 VL - 2015 AB - The immunosuppressive properties of mesenchymal stromal/stem cells (MSC) rendered them an attractive therapeutic approach for immune disorders and an increasing body of evidence demonstrated their clinical value. However, the influence of MSC on the function of specific immune cell populations, namely, monocyte subpopulations, is not well elucidated. Here, we investigated the influence of human bone marrow MSC on the cytokine and chemokine expression by peripheral blood classical, intermediate and nonclassical monocytes, and myeloid dendritic cells (mDC), stimulated with lipopolysaccharide plus interferon (IFN)γ. We found that MSC effectively inhibit tumor necrosis factor- (TNF-) α and macrophage inflammatory protein- (MIP-) 1β protein expression in monocytes and mDC, without suppressing CCR7 and CD83 protein expression. Interestingly, mDC exhibited the highest degree of inhibition, for both TNF-α and MIP-1β, whereas the reduction of TNF-α expression was less marked for nonclassical monocytes. Similarly, MSC decreased mRNA levels of interleukin- (IL-) 1β and IL-6 in classical monocytes, CCL3, CCL5, CXCL9, and CXCL10 in classical and nonclassical monocytes, and IL-1β and CXCL10 in mDC. MSC do not impair the expression of maturation markers in monocytes and mDC under our experimental conditions; nevertheless, they hamper the proinflammatory function of monocytes and mDC, which may impede the development of inflammatory immune responses. SN - 1687-966X UR - https://doi.org/10.1155/2015/819084 DO - 10.1155/2015/819084 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -