Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells

Figure 3

Influence of MSC on CCR7, CD83, and HLA-DR protein expression in monocytes and mDC. Percentage (mean ± standard deviation) of CCR7⁺ (a) and CD83⁺ (b) cells among classical, intermediate, and nonclassical monocytes and mDC, under the following culture conditions: nonstimulated MNC (MNC), nonstimulated MNC cocultured with MSC (MNC + MSC), MNC stimulated with LPS + IFN (MNC + LPS + IFN), MNC cocultured with MSC and stimulated with LPS + IFN in the presence of MSC (MNC + MSC + LPS + IFN), MNC cocultured with MSC and stimulated with LPS + IFN immediately after depletion of MSC from the culture system (MNC + MSC + Depletion + LPS + IFN). Statistically significant differences were considered when for Friedman’s paired-sample test: MNC; MNC + MSC. (c) Expression of HLA-DR (measured as mean fluorescence intensity) among CCR7⁺ and CCR7⁻ monocyte subpopulations and mDC. Statistically significant differences were considered when () for Friedman’s paired-sample test. MSC, mesenchymal stromal cells; mDC, myeloid dendritic cells; MNC, mononuclear cells; LPS, lipopolysaccharide; IFN, interferon .

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