Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells

Table 1

Panel of mAb reagents (with clones and commercial source) used for the immunophenotypic characterization of classical, intermediate, and nonclassical monocytes, mDC, and MSC.

Fluorochrome
Tube	PacB	PacO	FITC	PE	PerCPCy5.5	PECy7	APC	APCH7
	CD44	CD45	CD106	CD73	CD184	CD13	CD90
1	IM7	HI30	51-10C9	AD2	12G5	Immu103.44	5E10
	Biolegend	Invitrogen	BD Pharmingen	BD Pharmingen	BD	Beckman Coulter	BD Pharmingen
	CD16	CD45	CD83	CCR7	CD14	CD33	IREM-2	HLA-DR
2	3G8	HI30	HB15a	3D12	M5E2	D3HL60.251	UP-H2	L243
	BD Pharmingen	Invitrogen	Beckman Coulter	BD Pharmingen	BD Pharmingen	Beckman Coulter	Immunostep SL	BD
	CD16	CD45	TNF-α	MIP-1β	CD14	CD33	IREM-2	HLA-DR
3	3G8	HI30	MP6-XT22	D21-1351	M5E2	D3HL60.251	UP-H2	L243
	BD Pharmingen	Invitrogen	BD Pharmingen	BD Pharmingen	BD Pharmingen	Beckman Coulter	Immunostep SL	BD
	CD16			CD123	CD14	CD33	IREM-2	HLA-DR
4	3G8			SSDCL Y107D2	M5E2	D3HL60.251	UP-H2	L243
	BD Pharmingen			Beckman Coulter	BD Pharmingen	Beckman Coulter	Immunostep SL	BD

mAb, monoclonal antibody; mDC, myeloid dendritic cells; MSC, mesenchymal stromal cells; PacB, pacific blue; PacO, pacific orange; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCPCy5.5, peridinin chlorophyll protein-cyanine 5.5; PECy7, phycoerythrin-cyanine 7; APC, allophycocyanin; APCH7, allophycocyanin-hilite 7. Commercial sources: Biolegend (San Diego, CA, USA); Invitrogen, Life Technologies (Carlsbad, CA, USA); BD Pharmingen (San Diego, CA, USA); BD (Becton Dickinson Biosciences, San Jose, CA, USA), Beckman Coulter (Miami, FL, USA); Immunostep S.L (Salamanca, Spain).