In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells

Figure 5

Differentiation potential of cord tissue derived UC-MSCs into dermal fibroblast-like cells. Cytomorphology of (a) positive control, (b) untreated UC-MSCs, and (c) induced dermal fibroblast-like cells. Immunofluorescence staining with collagen-3, desmin, FAP-α, and procollagen-1 in ((d1)–(d4)) untreated UC-MSCs and in ((e1)–(e4)) induced cells. Expression of mRNA of collagen-3, desmin, FGF7, and vimentin has been shown in (f). Expression of these genes was significantly upregulated in induced cells as compared to untreated cells. Collagen-3: collagen type 3, FGF7: fibroblast growth factor 7, FAP-α: fibroblast activation protein-alpha, and procollagen-1: procollagen type 1. (g) showed graphical representation of RT-PCR analysis.

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