Research Article

Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis

Figure 2

Coculture increases proliferation of me-CH. (a) BrdU was stained for proliferating cells at day 3. Positive cells are shown in red, indicated by white arrowheads. Green cells are PKH67 labeled me-CH. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (). Data is shown as mean + standard deviation. Statistical significance was analyzed by Student’s -test. . (c) Immunofluorescent and BrdU staining for FGF1 is performed on SSCs pellet and coculture pellet. Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm.
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