Study on the Dynamic Biological Characteristics of Sca-1⁺ Hematopoietic Stem and Progenitor Cell Senescence

Figure 3

The changes of the SA-β-Gal staining in Sca-1⁺ HSPCs by aging. (a) The percentage of SA-β-gal positive cells in Sca-1⁺ HSPCs (%, , ). Group A: 3-4-week-old mice, 13–16 g in body weight. Group B: 8-9-week-old mice, 20–25 g in body weight. Group C: 6-month-old mice, 35–40 g in body weight. Group D: 12-month-old mice, 39–43 g in body weight. Group E: 18-month-old mice, 37–45 g in body weight. (a–d) represent a significant difference of these columns (). (b) Representative micrographs depict morphology of SA-β-gal positive cells and SA-β-gal negative cells in Sca-1⁺ HSPCs (×400). SA-β-gal positive cells are dyed in blue. Scale bars indicate 10 µm.

(a)

(b)