Dual Inhibition of Activin/Nodal/TGF-β and BMP Signaling Pathways by SB431542 and Dorsomorphin Induces Neuronal Differentiation of Human Adipose Derived Stem Cells

Figure 3

mRNA expression of neuronal markers in hADSCs upon dual inhibition of activin/nodal/TGF-β and BMP signaling pathways. hADSCs were cultured in BM or BM containing either activin/nodal/TGF-β pathway inhibitor SB or BMP pathway inhibitor DM or both for 14 and 21 days. The cells were harvested after 14 and 21 days to isolate RNA and prepare cDNA. Using gene specific primers and real-time PCR, expression of neuron cell specific transcription factors (Pax6 and Sox1), immature neuronal marker (tubulin IIIβ), and matured neuronal marker (NF200) was quantified. values are denoted compared to respective BM group.