Modulation of Synovial Fluid-Derived Mesenchymal Stem Cells by Intra-Articular and Intraosseous Platelet Rich Plasma Administration

Figure 3

Differentiation assay. In vitro differentiation assay of synovial fluid isolated cells to the mesenchymal lineages, adipocytes, osteoblasts, and chondrocytes. Control medium was expansion medium. Adipogenic differentiation was visualized by Oil Red (OR) staining. Osteogenic differentiation was visualized with Alizarin Red (AR) staining. Chondrogenic differentiation was visualized with Toluidine Blue (TB) staining and COL2 and immunohistochemistry using a monoclonal antibody directed to type II collagen. CTL, no primary antibody was added.