Stem Cells International / 2016 / Article / Fig 5

Research Article

Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

Figure 5

Expression of smooth muscle contractile markers was downregulated in induced hASCs transfected with siRNA against CaMKIIγ. (a) Expression of smooth muscle contractile markers in si-CaMKIIγ and si-control group determined by qRT-PCR. Data represent means ± SE, ( versus si-control). (b) Western blot analysis of smooth muscle contractile proteins in si-CaMKIIγ and si-control group, respectively. Data represent means ± SE, ( versus si-control). (c) Immunofluorescent staining of a-SMA, SM22a, and SM-MHC (red) in si-CaMKII and si-control group, respectively. Nuclear were stained with DAPI (blue). Scale bars: 100 μm.
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