Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells

Figure 2

Comparative analysis of the effect of pro- and anti-inflammatory cytokines on osteogenic differentiation of hASCs. ASCs were stimulated for 72 h with proinflammatory cytokines TNF-α, IL-6, IL-8, and IL-17F and the anti-inflammatory cytokine IL-4 (10 ng/mL). (a) IL-4 decreased RUNX2 gene expression at days 4 and 7, . (b) TNF-α and IL-6 decreased gene expression of COL1 at day 4 and 48 h, . (c) Only IL-4, but not the other cytokines tested, increased osteocalcin gene expression at days 4 and 7. Results are mean SD, . effect of cytokine treatment, .

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