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Stem Cells International
Volume 2016, Article ID 1329459, 9 pages
Research Article

The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation

1Division of Cardiology, Department of Medicine, Institute for Stem Cell Biology and Regenerative Medicine and Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
2CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary’s Hospital, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of Korea
3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, Institute of Medical Science, College of Medicine, Catholic University of Korea, Seoul 137-701, Republic of Korea

Received 2 February 2016; Revised 8 June 2016; Accepted 3 July 2016

Academic Editor: Eva Mezey

Copyright © 2016 Youngkyun Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues. We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs. By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs. The generated iPSCs expressed pluripotent markers and differentiated into all three germ layer lineages. The protocol can also be used with umbilical cord blood mononuclear cells (CBMCs). In this study, we present a simple and efficient protocol that improved the yield of iPSCs from floating cells such as PBMCs and CBMCs by serial plating and centrifugation.