Stem Cells International / 2016 / Article / Fig 1

Research Article

A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing

Figure 1

Analysis pipeline of sequencing data. Identification of variants was performed using two parallel analysis tools: Ion Torrent Variant Caller Plugin version 4.0 (Life Technologies) with visual inspection of the read alignment on the Integrative Genomics Viewer (IGV, Broad Institute, Boston, MA) and NextGENe software package version 2.3.4 (SoftGenetics, State College, PA) with the read alignment directly inspected on the NextGENe Viewer.

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