Construction of a Dual-Fluorescence Reporter System to Monitor the Dynamic Progression of Pluripotent Cell Differentiation

Figure 2

Promoter activity assay for the pig Oct4 promoter. (a) Four EGFP reporter vectors carrying different lengths of the pig Oct4 URS were constructed from a pEGFP-C2 vector. DE: distal enhancer; PE: proximal enhancer. PP: Pou5f1 proximal promoter. The deleted regions are shown in gray. (b) The removal of the 2.5 kb 5′-flanking region (pOg2 vector) had no significant impact () on the promoter activity in each cell line. However, the promoter activity was significantly decreased in the F9 cell line () when the DE region was removed. An obvious drop of promoter activity was detected in the P19 cell line () when the PE region was removed. Relative activity of each promoter construct is reflected by fluorescence intensity. Data are represented as the mean ± SEM. indicates value < 0.05 versus normal. indicates value < 0.01 versus normal. Similar results were observed in more than two independent experiments.

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