Construction of a Dual-Fluorescence Reporter System to Monitor the Dynamic Progression of Pluripotent Cell Differentiation

Table 1

List of primers used in this study.

Primers		Primer length (bp)	Amplicon size (bp)
2.5Up-F	5′-attaatTGTGAGCACAGTTCCATCCTGACC -3′	30	2526
2.5Up-R	5′-attaatCCAGCTGAAATGACTCCTGGGGAA -3′	30
DE1-F	5′-GCTTGTCCTTAAGGTTCTGGGTCA-3′	24	351
DE1-R	5′-gtcgacATCTACTGCTGAGCTCCTTGGCTC-3′	30
DE2-F	5′-gtcgacGAAGCACATCTTTCCACCCCCACC-3′	30	683
DE2-R	5′-CTCCTCTGAATCTCTTCCAGTGCC-3′	24
PE1-F	5′-TTTTCGCTAGCCCCCCAAACAAAG-3′	24	164
PE1-R	5′-gtcgacTCACACAGAATCCCCTTCAGAGCA-3′	30
PE2-F	5′-gtcgacTCTCCCCCCCACCTCCCTCCTT-3′	28	759
PE2-R	5′-GGTGTCTCGAGGGCGAAAGTCGGA-3′	24
AscI adaptor	5′-TAATggcgcgccAT-3′	14	—

enzyme sites are shown in lowercase. F: forward; R: reverse; DE: distal enhancer; PE: proximal enhancer.