Research Article
Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells
Figure 5
LO-EPC adhesion to various extracellular matrix proteins under static and flow conditions. 2 × 104 of LO-EPC were plated to Ibidi μ-Slide VI 0.4 Luer slides for 45 min to study the static adhesion of LO-EPC to various ECM (a). Dynamic adhesion of LO-EPC to various extracellular matrix proteins under 0.7 dyn/cm2 shear stress (b). 4 × 105 EPC were perfused into Ibidi μ-Slide VI 0.4 Luer slides coated with 100 μg/mL collagen IV, collagen I, fibronectin, vitronectin, and laminin, respectively. The data was represented as mean ± SE of three experiments. compared to fibronectin.
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