Research Article
Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells
Figure 7
The influence of fibronectin on the dynamic interaction of LO-EPC with control HAEC and HAEC with ischaemia reperfusion injury under 0.7 dyn/cm2 shear stress. 3 × 104 HAEC were plated into Ibidi μ-Slide VI 0.4 Luer slides which had been precoated with 100 μg/mL fibronectin. The data represent a mean ± SE of three experiments. LO-EPC adhesion to fibronectin was integrin dependent (b). LO-EPC binding to fibronectin was blocked by antibodies against integrin α5β1, integrin αvβ1, and integrin αvβ3. The data represent the mean ± SE of three experiments. compared to the control (no blocking antibodies).
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