Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes

Figure 4

Characterization of isolated BM-MSCs from different aged C57BL/6 mice. (a) Bone marrow abundance was determined by CFU assay. Data are mean ± SEM (). : young versus old. (b) Morphology from MSC derived from different age groups. Scale bar 100 μm. (c) Proliferation kinetics was evaluated by crystal violet staining (570 nm absorbance) over a period of 12 days. Data are mean ± SEM (). (d) Differentiation potential was assessed by in vitro exposure to adipogenic or osteogenic medium and stained with Oil Red and Alizarin Red after 21 days, respectively. (e) Flow cytometry was used for the assessment of MSC surface markers. MSCs expressed anti-CD90.2, anti-Sca-1, and anti-ASMA antibodies but not anti-CD45.2 and anti-CD11b antibodies. Representative images for 2 animals per each age group. Representative images from 3 animals per each age group. Scale bar 100 μm. (f) The mRNA profiles of MSC paracrine factors (VEGFα, VEGFδ, ANG-I, ANG-II, IGF-1, KGF, HGF, CoL-1, MMP-1, and MMP-2) from different aged donors were analyzed by RT-PCR and normalized by their relative ratio to GAPDH. Data are mean ± SEM (). GAPDH, glyceral-dehyde-3-phosphate dehydrogenase. .

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