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Stem Cells International
Volume 2016, Article ID 1461648, 15 pages
Research Article

Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes

1Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, Chile
2Production Unity of Advanced Therapy, Fundación Oftalmológica de Santander, Clínica Carlos Ardila Lulle (Foscal Internacional), Universidad Autónoma de Bucaramanga (UNAB), 681004153 Bucaramanga, Colombia

Received 30 December 2015; Accepted 1 March 2016

Academic Editor: Laura Lasagni

Copyright © 2016 Flavia Bruna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: We standardized histological criteria in haematoxylin-eosin to provide quantitative analysis regarding the dermoepidermal junction and degree of leukocyte infiltration.

Supplementary Figure 2: We evaluate reepithelialization with an epithelial tissue marker (pan-cytokeratin). We found expression in all groups.

Supplementary Figure 3: We evaluate granulation tissue that is a tissue of neoformation with reparative properties. We observed macroscopically higher granulation tissue formation on day 4 in the bed of BM-MSCs-treated wounds than wounds treated with acd-MSCs or vehicle alone.

Supplementary Table 1: Statistical data of flow cytometry showed that no significant age-related changes in the marker expression levels between the three age groups.

Supplementary Table 2: List with the specific primer.

  1. Supplementary Material