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Stem Cells International
Volume 2016 (2016), Article ID 1580701, 16 pages
Research Article

Lovastatin Decreases the Expression of CD133 and Influences the Differentiation Potential of Human Embryonic Stem Cells

Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia

Received 8 March 2016; Accepted 18 April 2016

Academic Editor: Mario Cioce

Copyright © 2016 Ade Kallas-Kivi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

S Figure 1. Expression of differentiation markers and the pluripotency marker OCT4 in hES cells and formed embryoid bodies. hES cells were treated with 20 μM lovastatin for 48 h, then a sample of the hES cells were analyzed for the expression of differentiation markers and OCT4, and EB were formed from another cell sample.

S Figure 2. Morphological changes in the colony structures of untreated and lovastatin treated hES cells during differentiation into ectodermal, mesodermal or endodermal lineages.

S Figure 3. Characterisation of CD133+ and CD133- hES cell subpopulations obtained using the magnetic beads separation (MACS) method. (A) Expression of CD133, OCT4, SSEA3 and SOX2 in these cell populations are displayed as dot plots or (B) histograms that show the distribution of cells in terms of the various expression levels of specific markers.

  1. Supplementary Figures