Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Figure 3

Acute electrical stimulation enhances cardiac differentiation of iPS(Foreskin)-2 cell line. (a-b) The effect of acute electrical stimulation at 65 mV/mm (a) or 200 mV/mm (b) on cardiac differentiation of human iPS(Foreskin)-2 cell line (–15 independent experiments). (c-d) Quantitative RT-PCR analysis for the expression of cardiac transcription factors (c) and cardiac contractile muscle proteins (d) in electrically stimulated and control EBs at days 0, 1, and 7 after electrical stimulation ( independent experiments). Data are expressed as mean ± SEM. , , and by one-way (a-b) or two-way (c-d) ANOVA with the Bonferroni post hoc test.

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