Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Figure 5

Expression of cardiac-specific markers in cardiomyocytes derived from iPS(Foreskin)-2 cell line. (a) Beating EBs expressed cardiac contractile proteins, cardiac troponin T, sarcomeric α-actinin, myosin heavy chain, and gap junctional protein connexin 43 (Cx43, white arrow) (scale bar = 50 μm). Quantitative RT-PCR analysis for the expression of cardiac transcription factors (b) and cardiac contractile muscle proteins (c) in electrically stimulated and control beating EBs at day 14 after plating (–7 independent experiments). (d) MYL2/MYL7 gene expression ratio. (e) Percentage of cardiac troponin T-positive cells in each beating EB at day 14 after plating (-6 independent experiments). Data are expressed as mean ± SEM. ES: electrically stimulated and undiff: undifferentiated iPSCs.

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