Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study

Figure 4

Effects of storage solution and storage time on cell clumping and viability before transplantation. Fresh passage 5 rat BMMSCs were resuspended in complete growth medium, normal saline, and DPBS and stored at 37°C for 3 h, 6 h, and 9 h before analysis. (a) Cell clumps or large cells >30 µm; (b) viable cells; (c) dead cells; (d) apoptotic cells. normal saline versus growth medium. normal saline versus growth medium. DPBS versus growth medium; DPBS versus growth medium; DPBS versus growth medium.

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