Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

Figure 4

Gene expression of the vasculogenic differentiation markers CD31, VEGF165 and VEGF189, and EDN1 in BCP60/40- and BCP20/80-based composites, and fibrin gels at days 1, 7, and 11 of culture. (a) BCP20/80-based composites showed increased CD31 gene expression compared to BCP60/40-based composites at day 11, as well as to fibrin gels. (b) BCP60/40- and BCP20/80-based composites demonstrated similar VEGF165 gene expression at all time points. (c) BCP20/80-based composites showed increased VEGF189 gene expression compared to BCP60/40-based composites at days 1 and 7. (d) BCP60/40- and BCP20/80-based composites showed similar EDN1 gene expression at all time points. Values are mean ± SEM (–12).Significant effect of BCP60/40- or BCP20/80-based composites compared to fibrin gels, .Significantly different from BCP60/40-based composites, . BCP, biphasic calcium phosphate; CD31, cluster of differentiation-31; VEGF165, vascular endothelial growth factor-165; VEGF189, vascular endothelial growth factor-189; EDN1, endothelin-1.

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