Stem Cells International / 2016 / Article / Tab 2

Research Article

Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

Table 2

Primer sequences for determination of osteogenic and vasculogenic differentiation potential through PCR. YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; KI67: proliferation marker; RUNX2: runt-related transcription factor-2; ON: osteonectin; DMP1: dentin matrix acidic phosphoprotein-1; COL1: collagen-1; COL3: collagen-3; CD31: cluster of differentiation-31; VEGF165: vascular endothelial growth factor-165; VEGF189: vascular endothelial growth factor-189; EDN1: endothlin-1; bp: base pairs.

Target geneOligonucleotide sequenceProduct size (bp)Annealing temperature (°C)

YWHAZForward5′ gATgAAgCCATTgCTGAACTTg 3′22956
Reverse5′ CTATTTgTgggACAgCATggA 3′

KI67Forward5′ CCCTCAgCAAgCCTgAgAA 3′20256
Reverse5′ AgAggCgTATTAggAggCAAg 3′

RUNX2Forward5′ ATgCTTCATTCgCCTCAC 3′15656
Reverse5′ ACTgCTTgCAgCCTTAAAT 3′

ONForward5′ CTgTCCAggTggAAgTAgg 3′23356
Reverse5′ gTggCAggAAgAgTCgAAg 3′

DMP1Forward5′ TAggCTAgCTggTggCTTCT 3′37556
Reverse5′ AACTCggAgCCgTCTCCAT 3′

COL1Forward5′ TCCggCTCCTgCTCCTCTTA 3′33656
Reverse5′ ggCCAgTgTCTCCCTTg 3′

COL3Forward5′ gATCCgTTCTCTgCgATgAC 3′27956
Reverse5′ AgTTCTgAggACCAgTAggg 3′

CD31Forward5′ AACAggAgggAgAgTATTACTg 3′23656
Reverse5′ TggTACTgCTggCCTggA 3′

VEGF165Forward5′ ATCTTCAAgCCATCCTgTgTgC 3′22456
Reverse5′ CAAggCCCACAgggATTTTC 3′

VEGF189Forward5′ ATCTTCAAgCCATCCTgTgTgC 3′28956
Reverse5′ CACAgggAACgCTCCAggAC 3′

EDN1Forward5′ gTTTgTggCTTgCCAAggA 3′20756
Reverse5′ ACgTgCTCgggAgTgTTgA 3′

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