Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

Table 2

Primer sequences for determination of osteogenic and vasculogenic differentiation potential through PCR. YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; KI67: proliferation marker; RUNX2: runt-related transcription factor-2; ON: osteonectin; DMP1: dentin matrix acidic phosphoprotein-1; COL1: collagen-1; COL3: collagen-3; CD31: cluster of differentiation-31; VEGF165: vascular endothelial growth factor-165; VEGF189: vascular endothelial growth factor-189; EDN1: endothlin-1; bp: base pairs.

Target gene		Oligonucleotide sequence	Product size (bp)	Annealing temperature (°C)
YWHAZ	Forward	5′ gATgAAgCCATTgCTGAACTTg 3′	229	56
	Reverse	5′ CTATTTgTgggACAgCATggA 3′
KI67	Forward	5′ CCCTCAgCAAgCCTgAgAA 3′	202	56
	Reverse	5′ AgAggCgTATTAggAggCAAg 3′
RUNX2	Forward	5′ ATgCTTCATTCgCCTCAC 3′	156	56
	Reverse	5′ ACTgCTTgCAgCCTTAAAT 3′
ON	Forward	5′ CTgTCCAggTggAAgTAgg 3′	233	56
	Reverse	5′ gTggCAggAAgAgTCgAAg 3′
DMP1	Forward	5′ TAggCTAgCTggTggCTTCT 3′	375	56
	Reverse	5′ AACTCggAgCCgTCTCCAT 3′
COL1	Forward	5′ TCCggCTCCTgCTCCTCTTA 3′	336	56
	Reverse	5′ ggCCAgTgTCTCCCTTg 3′
COL3	Forward	5′ gATCCgTTCTCTgCgATgAC 3′	279	56
	Reverse	5′ AgTTCTgAggACCAgTAggg 3′
CD31	Forward	5′ AACAggAgggAgAgTATTACTg 3′	236	56
	Reverse	5′ TggTACTgCTggCCTggA 3′
VEGF165	Forward	5′ ATCTTCAAgCCATCCTgTgTgC 3′	224	56
	Reverse	5′ CAAggCCCACAgggATTTTC 3′
VEGF189	Forward	5′ ATCTTCAAgCCATCCTgTgTgC 3′	289	56
	Reverse	5′ CACAgggAACgCTCCAggAC 3′
EDN1	Forward	5′ gTTTgTggCTTgCCAAggA 3′	207	56
	Reverse	5′ ACgTgCTCgggAgTgTTgA 3′