Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells

Table 1

IEF conditions for 11 cm IPG (3–10).

Step number	Voltage	Voltage ramping mode	Time
Step  1	150 V	Linear	1 hour
Step  2	300 V	Linear	2.30 hours
Step  3	600 V	Linear	1.50 hours
Step  4	1200 V	Linear	1.50 hours
Step  5	4000 V	Slow	1.50 hours
Step  6	8000 V	Slow	1 hour
Step  7	8000 V	Linear	30000 volt-hours
Step  8	500 V	Slow	0.15 hours