Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids

<div>Alizarin red stain quantified by measurement of calcium deposits. (a) hDPSCs were cultured for 7, 14, or 21 days in the presence of one of the 3 conditioned media described under Materials and Methods and based on 0,01 M melatonin (medium M) or a mixture of HA 2 mg/mL + BU 5 mM + RA 1 <i>μ</i>M (medium H) or on a combination of melatonin together with HA + BU + RA (medium MH) or kept under an undifferentiated state by growing on medium C. Scale bar = 200 um. (b) Quantitative mineralization of hDPSCs cultured in maintenance medium (medium C) or the different osteogenic media (medium M, medium H, and medium MH). Results are expressed as mean + SD. An average was made from three technical replicates. Values represent the mean standard deviation of 4 different experiments.</div>

Stem Cells International

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Figure 4: Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids 

Figure 4 | Osteogenesis from Dental Pulp Derived Stem Cells: A Novel Conditioned Medium Including Melatonin within a Mixture of Hyaluronic, Butyric, and Retinoic Acids 