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Stem Cells International
Volume 2016, Article ID 2059584, 5 pages
http://dx.doi.org/10.1155/2016/2059584
Research Article

Identification of Stem-Like Cells in Atrial Myxoma by Markers CD44, CD19, and CD45

1Department of Cardiology, Rizhao Hospital of Traditional Chinese Medicine, Shandong 276800, China
2Nankai University School of Medicine, Tianjin 300071, China
3Department of Cardiology, Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin 300121, China
4Department of Intensive Care Unit (ICU), People’s Hospital of Rizhao, Shandong 276826, China
5Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China
6The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, The College of Life Science, Tianjin 300071, China

Received 6 November 2016; Accepted 8 December 2016

Academic Editor: Jijun Hao

Copyright © 2016 Xianghe Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Atrial myxoma is the most frequent tumor arising mainly in atrial septum and its origin remains uncertain. It has been reported that a subpopulation of stem-like cells are present in benign tumors and responsible for tumor initiation and maintenance. In this study, we investigated whether stem-like cells could contribute to the atrial cardiac myxoma. Immunohistology data confirmed that a population of cells bearing the surface markers CD19, CD45, and CD44 resided in a mucopolysaccharide-rich matrix of myxoma. Moreover, we isolated myxoma cells with phase-bright culture method and confirmed that myxoma derived cells express robust level of CD19, CD45, and CD44. Furthermore, the pluripotency of this population of cells also was validated by cardiomyocytes and smooth muscle cells differentiation in vitro. Our results indicate that primary cardiac myxoma may arise from mesenchymal stem cells with the ability to generate tumors with multilineage differentiation. In conclusion, this study for the first time verified that stem-like cells are present in atrial myxoma and this population of cells may have the capacity for myxoma initiation and progression.