Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner

Figure 2

Phenotypic characteristics of expanded MSCs. (a) Cells with fibroblast-like morphology attached to the culture plate after the first passage of exocrine cells and maintained a similar morphology throughout continuous passages 1 through 12. (b) Passage six hpMSCs were fixed with 4% PFA and underwent immunofluorescence staining with anti-insulin, glucagon, amylase, and CA19-9. Pancreatic cell hormone insulin, glucagon, amylase, and enzymes from exocrine cells were not detected, whereas CA19-9 was detected in most cells. (c) Flow cytometry analysis of fibroblast-like cells harvested at passage day 6. hMSC markers were labeled using PE-conjugated antibodies against isotypes CD73, CD90, CD105, CD14, CD19, CD45, CD31, MCAM, HCAM, αSMA, or FITC-conjugated CA19-9. hMSC-positive markers CD73 (96.7%), CD90 (93.5%), CD105 (91%), αSMA (40.4%), MCAM (98.4%), and HCAM (86%) were detected, and hMSC-negative markers CD14, CD19, CD45, and CD31 were not. Pancreatic duct cells positive for CA19-9 (96.9%) were also detected. (d) hMSC-phenotype markers confirmed by immunofluorescence staining. CD90, CD105, MCAM, and HCAM exhibited the strongest signals, whereas CD14 and CD19 were not detected during passage six.

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