Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2016, Article ID 2152435, 15 pages
http://dx.doi.org/10.1155/2016/2152435
Research Article

The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells

1Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
2Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland
3Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland
4Department of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, Poland

Received 2 August 2015; Revised 31 December 2015; Accepted 5 January 2016

Academic Editor: Christian Dani

Copyright © 2016 Monika Marędziak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potential of mesenchymal stem cells. The aim of this study was to assess the age-related maintenance of physiological function and differentiation potential of hASCs in vitro. hASCs were isolated from patients of four different age groups: (1) >20 years (), (2) >50 years (), (3) >60 years (), and (4) >70 years (). The hASCs were characterized according to the number of fibroblasts colony forming unit (CFU-F), proliferation rate, population doubling time (PDT), and quantified parameters of adipogenic, chondrogenic, and osteogenic differentiation. Compared to younger cells, aged hASCs had decreased proliferation rates, decreased chondrogenic and osteogenic potential, and increased senescent features. A shift in favor of adipogenic differentiation with increased age was also observed. As many bone and joint diseases increase in prevalence with age, it is important to consider the negative influence of age on hASCs viability, proliferation status, and multilineage differentiation potential when considering the potential therapeutic applications of hASCs.