Research Article
The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells
Table 1
Primer sequences used in qPCR.
| | Gene | Primer 5′-3′ | Amplicon length (bp) | Accession number |
| | ACAN | F: GCCTACGAAGCAGGCTATGA | 136 | NM_013227.3 | | R: GCACGCCATAGGTCCTGA |
| | ADIQ | F: AGGGTGAGAAAGGAGATCC | 4155 | XM_011513324.1 | | R: GGCATGTTGGGGATAGTAA |
| | BMP-2 | F: ATGGATTCGTGGTGGAAGTG | 349 | KC294426.1 | | R: GTGGAGTTCAGATGATCAGC |
| | COL-I | F: GTGATGCTGGTCCTGTTGGT | 123 | NM_000088.3 | | R: CACCATCGTGAGCCTTCTCT |
| | COL-II | F: GACAATCTGGCTCCCAAC | 257 | NM_001844.4 | | R: ACAGTCTTGCCCCACTTAC |
| | GAPDH | F: GTCAGTGGTGGACCTGACCT | 286 | NM_0017008.4 | | R: CACCACCCTGTTGCTGTAGC |
| | LEP | F: ATGACACCAAAACCCTCATCAA | 22 | XM_005250340.3 | | R: GAAGTCCAAACCGGTGACTTT |
| | OCL | F: ATGAGAGCCCTCACACTCCTC | 292 | NM_199173.4 | | R: CGTAGAAGCGCCGATAGGC |
| | OPN | F: AAACGCCGACCAAGGTACAG | 213 | U20758.1 | | R: ATGCCTAGGAGGCAAAAGCAA |
| | PPARγ | F: ATGACACCAAAACCCTCATCAA | 125 | AB565476.1 | | R: GAGCGGGTGAAGACTCATGTCTGTC |
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