Research Article
Utility of Lymphoblastoid Cell Lines for Induced Pluripotent Stem Cell Generation
Table 1
iPSC induction efficiency from six LCL lines.
| Cell line | Cell number nucleofected | Reprogramming efficiency (%)/plasmid mixture and conditions | EN2L + ET2K + EM2K after Choi et al. [15] | OSNK + OSTK + L-mL after Rajesh et al. [16] | Our optimized protocol (see Materials and Methods) |
| LCL-1 | 1 × 106 | 0.0 | 0.0003 | 0.0102 | LCL-2 | 1 × 106 | 0.0 | 0.0 | 0.0204 | LCL-3 | 1 × 106 | 0.0 | 0.0001 | 0.0054 | LCL-4 | 1 × 106 | 0.0 | 0.0 | 0.0108 | LCL-5 | 1 × 106 | 0.0 | 0.0 | 0.0132 | LCL-6 | 1 × 106 | 0.0 | 0.0 | 0.0216 |
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