High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis

Figure 4

High magnification images of drug induced neutral lipid accumulation in droplets of hiPS derived hepatocytes (LipidTox Green) by amiodarone 12,5 μM (a), doxycycline 32 μM (b), tetracycline 100 μM (c), and cyclosporine A 30 μM (positive control, d) and drug induced phospholipidosis (LipidTox Red) by amiodarone 12,5 μM (e) and propranolol 30 μM (positive control, f). Nuclei are stained blue by DAPI. Scale bar 50 μM.

(a)

(b)

(c)

(d)

(e)

(f)