Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary

Figure 2

Transcriptome analysis of OCCs. (a) Principal component analysis of the transcriptome analyses of ovarian cell colonies (OCCs), neonatal ovaries, which served as starting material for OCC cultures, embryonic stem cells, and fibroblasts. The latter two served as reference samples. OCC transcriptomes differ from the neonatal ovaries’ transcriptomes. However, ovaries, ES cells, and OCCs are more similar among each other than to fibroblasts (see component 1, relative weight of 38.54%). (b) Top 50 differentially expressed genes between native neonatal ovary and OCCs. For gene bank and Ensembl identifiers, see Table  1 of Supplementary Material. (c) Top 50 differentially expressed genes between OCCs and fibroblasts. (d) Top 50 differentially expressed genes between OCCs and ES cells. (e) Gene ontology analysis of OCCs versus native ovary. Cell adhesion, ion and neurotransmitter transporters, and signaling pathways are predominantly upregulated in OCCs compared to the native ovary.

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