Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary

Figure 6

Characterization of the VASA antibody and detection of VASA-positive cells in OCCs. (a) Western blot analysis using marmoset monkey protein samples demonstrates the specificity of the VASA antibody. indicates that the ovary was from an aged animal. The ovarian germ cell pool was therefore most likely strongly reduced or even exhausted leading to undetectable VASA protein concentrations in the sample. (b and c) Immunohistochemical application of the VASA antibody to marmoset gonads showed germ-cell-specific labeling in the adult testis and the adult ovary. (d) DAPI staining of a part of an OCC. Note the intensely stained nucleus in the central part. (e) The same area shown in (d). The large cell containing the intensely stained nucleus strongly stains for VASA. The signal is blurry due to the fact that the picture was taken through the bottom of a normal cell culture dish. (f, g) Examples of isolated VASA-positive cells in sections of paraffin-embedded OCCs.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)