Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary

Figure 7

Pluripotency and germ cell marker mRNA abundance during the development of OCCs within one passage as revealed by real-time quantitative PCR. Positive controls for OCT4A, SALL4, and LIN28 were ES cells. Neonatal ovary was used as positive control for the germ cell marker VASA. NANOG was very low and OCT4A and LIN28 were absent at all time points analyzed. Relative abundance of SALL4 and VASA generally decreased with time during the culture period. D: days of culture. W: well for cell culture. NB: newborn ovary.

(a)

(b)

(c)

(d)

(e)