Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells

Figure 4

Effect of PL-MSCs conditioned medium fractions on EPC migration. (a) Diagram of the transwell culture system for an in vitro migration assay. (b) Hematoxylin stained EPCs which migrated to the other side of membrane in response to soluble factors presented in the 100 kDa, 50 kDa, 30 kDa, 10 kDa, and less than 10 kDa fractions of the PL-MSCs conditioned medium (scale bar = 200 μm). (c) Numbers of EPCs which migrated in response to soluble factors presented in the 100 kDa, 50 kDa, 30 kDa, 10 kDa, and less than 10 kDa fractions of PL-MSCs conditioned medium. EPCs cultured in transwells whose lower chamber contained serum-free medium (SFM) served as negative controls while EPCs cultured in transwells whose lower chamber contained unfractionated PL-MSCs conditioned medium served as positive controls. Data were presented as mean ± SEM of three independent experiments. Nonparametric Kruskal-Wallis test was used to assess the significance of differences between observed data. versus negative control.

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