The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial-Like Cells

Figure 2

CD163 overexpression promoted BMSC differentiation into vascular endothelial-like cells with or without ABO treatment. In control group, BMSCs were transfected with empty vector pCMV6 and treated with 0.05% DMSO for 48 h; in pCMV6-CD163 group, BMSCs were transfected with pCMV6-CD163 for 48 h; in ABO group, BMSCs were treated with 50 μM ABO for 48 h (DMSO as control); in pCMV6-CD163 + ABO group, BMSCs were transfected with pCMV6-CD163 and treated with 50 μM ABO for 48 h; and in HUVEC group, human umbilical vascular endothelial cells are considered as a positive control. (a) Immunostaining of the endothelial-function associated marker endothelial nitric oxide synthase (eNOS) in BMSCs treated as described above. Scale bar: 10 μm. (b) The relative fluorescent intensity was calculated and the fluorescent intensity of HUVECs was set as 100%. (c) Immunostaining of the endothelial-specific marker VE-cadherin in BMSCs treated as described above. Scale bar: 10 μm. (d) The relative fluorescent intensity was calculated and the fluorescent intensity of HUVECs was set as 100%. (e) The vascular structure formed on Matrigel by BMSCs treated as described above. Scale bar: 100 μm. Images are representatives of at least 3 independent experiments. Data are mean ± SEM. , , .

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