Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo

Figure 1

High expression of Sema3A correlates with the potent osteogenic capacity in BMSCs. (a) ASCs and BMSCs from the same rat were cultured in the osteogenic medium and significantly less mineralization nodules were found in ASCs than that in BMSCs after 21 days of osteogenic induction. (b) Quantification data of ECM mineralization in Figure 1(a). (c) Sema3A expression was significantly less in ASCs than that in BMSCs, as detected by qPCR. (d) Lower Sema3A expression was also found in 12-month rat derived BMSCs, compared with that from 4-week rat derived BMSCs. Mean ± SD, , and . Sema3A, semaphorin 3A; ECM, extracellular matrix.

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