Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo

Figure 2

Sema3A promotes osteogenic differentiation while inhibiting adipogenic differentiation in ASCs. (a) ASCs were infected with either empty pWPI or Sema3A overexpression lentivirus, respectively. showed higher ALP activity than after 7-day osteogenic induction. (b) Quantification data of ALP activity. (c) Significantly more mineralization nodules were found in group than that in group after 21 days of osteogenic induction. (d) Quantification data of ECM mineralization in Figure 2(c). (e) Sema3A significantly reduced lipid droplet formation in ASCs after 9 days of adipogenic induction. (f) Quantification data of lipid droplets in Figure 2(e). (g, h, and i) Expression of the osteogenesis related genes including ALP, RUNX2, and COL-1 after 5 and 10 days of osteogenic induction. (j, k, and l) Expression of the adipogenesis related genes including FABP4, PPARG, and C/EBPA after 3 and 5 days of adipogenic induction. Mean ± SD, , and .

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