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Stem Cells International
Volume 2016 (2016), Article ID 2545214, 13 pages
Research Article

Semaphorin 3A Shifts Adipose Mesenchymal Stem Cells towards Osteogenic Phenotype and Promotes Bone Regeneration In Vivo

1State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an 710032, China
2Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi’an, China
3Department of Plastic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, China
4Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, China

Received 18 June 2016; Revised 17 August 2016; Accepted 18 August 2016

Academic Editor: Giacomina Brunetti

Copyright © 2016 Xiangwei Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary data include three figures and one table. Supplementary Figure 1 refers to the detailed expression of semaphorin family members (Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G, 5A, 5B, 6A, 6B, 6C, 6D, and 7A) in ASCs and BMSCs and in BMSCs from different age groups. Supplementary Figure 2 depicts the overexpression efficiency of Sema3A as confirmed by flow cytometry, Q-PCR and Western blot. Supplementary Figure 3 refers to the role of Sema3A in BMSC osteogenesis via knockdown of Sema3A. Supplementary Table 1 covers all the primers used in the study.

  1. Supplementary Material