Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2016 (2016), Article ID 2562718, 9 pages
http://dx.doi.org/10.1155/2016/2562718
Research Article

Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression

1Cell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, Brazil
2Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Avenida Coronel Francisco Heráclito dos Santos 210, 81531-970 Curitiba, PR, Brazil
3Hospital Santé Curitiba, Plastic Surgery Clinic, Rua XV de Novembro 2913, 80045-340 Curitiba, PR, Brazil
4Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, Brazil
5Center for Translational Medicine, Temple University School of Medicine, Philadelphia, PA 19140, USA
6Flow Cytometric Analysis Laboratory, Clinical Hospital of Federal University of Paraná, Rua Padre Camargo 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil

Received 16 October 2015; Revised 5 December 2015; Accepted 15 December 2015

Academic Editor: Hiroshi Mizuno

Copyright © 2016 Ana Carolina Irioda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. Kim and S.-G. Cho, “Clinical applications of mesenchymal stem cells,” Korean Journal of Internal Medicine, vol. 28, no. 4, pp. 387–402, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. X. Wei, X. Yang, Z.-P. Han, F.-F. Qu, L. Shao, and Y.-F. Shi, “Mesenchymal stem cells: a new trend for cell therapy,” Acta Pharmacologica Sinica, vol. 34, no. 6, pp. 747–754, 2013. View at Publisher · View at Google Scholar · View at Scopus
  3. M. Ogawa, “Differentiation and proliferation of hematopoietic stem cells,” Blood, vol. 81, no. 11, pp. 2844–2853, 1993. View at Google Scholar · View at Scopus
  4. B. Logan, E. Leifer, C. Bredeson et al., “Use of biological assignment in hematopoietic stem cell transplantation clinical trials,” Clinical Trials, vol. 5, no. 6, pp. 607–616, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. F. De Francesco, G. Ricci, F. D'Andrea, G. F. Nicoletti, and G. A. Ferraro, “Human adipose stem cells: from bench to bed-side,” Tissue Engineering Part B: Reviews, vol. 21, no. 6, pp. 572–584, 2015. View at Publisher · View at Google Scholar
  6. I. A. Panfilov, R. Jong, S. Takashima, and H. J. Duckers, “Clinical study using adipose-derived mesenchymal-like stem cells in acute myocardial infarction and heart failure,” in Cellular Cardiomyoplasty, vol. 1036 of Methods in Molecular Biology, pp. 207–212, Humana Press, 2013. View at Publisher · View at Google Scholar
  7. P. A. Zuk, M. Zhu, H. Mizuno et al., “Multilineage cells from human adipose tissue: implications for cell-based therapies,” Tissue Engineering, vol. 7, no. 2, pp. 211–228, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. P. A. Zuk, M. Zhu, P. Ashjian et al., “Human adipose tissue is a source of multipotent stem cells,” Molecular Biology of the Cell, vol. 13, no. 12, pp. 4279–4295, 2002. View at Publisher · View at Google Scholar · View at Scopus
  9. B. A. Bunnell, M. Flaat, C. Gagliardi, B. Patel, and C. Ripoll, “Adipose-derived stem cells: isolation, expansion and differentiation,” Methods, vol. 45, no. 2, pp. 115–120, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. A. De Rosa, F. De Francesco, V. Tirino et al., “A new method for cryopreserving adipose-derived stem cells: an attractive and suitable large-scale and long-term cell banking technology,” Tissue Engineering - Part C: Methods, vol. 15, no. 4, pp. 659–667, 2009. View at Publisher · View at Google Scholar · View at Scopus
  11. K. A. T. Carvalho, C. C. Cury, L. Oliveira et al., “Evaluation of bone marrow mesenchymal stem cell standard cryopreservation procedure efficiency,” Transplantation Proceedings, vol. 40, no. 3, pp. 839–841, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. P. A. Sotiropoulou, S. A. Perez, M. Salagianni, C. N. Baxevanis, and M. Papamichail, “Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells,” Stem Cells, vol. 24, no. 2, pp. 462–471, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. D. A. De Ugarte, Z. Alfonso, P. A. Zuk et al., “Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow,” Immunology Letters, vol. 89, no. 2-3, pp. 267–270, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. K. A. T. Carvalho, R. B. Simeoni, L. C. Guarita-Souza et al., “Angiogenesis without functional outcome after mononuclear stem cell transplant in a doxorubicin-induced dilated myocardiopathy murine model,” The International Journal of Artificial Organs, vol. 31, no. 5, pp. 431–438, 2008. View at Google Scholar · View at Scopus
  15. B. C. Goh, S. Thirumala, G. Kilroy, R. V. Devireddy, and J. M. Gimble, “Cryopreservation characteristics of adipose-derived stem cells: maintenance of differentiation potential and viability,” Journal of Tissue Engineering and Regenerative Medicine, vol. 1, no. 4, pp. 322–324, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. J. B. Mitchell, K. Mcintosh, S. Zvonic et al., “Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell–associated markers,” STEM CELLS, vol. 24, no. 2, pp. 376–385, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. R. J. Ferreira, A. C. Irioda, R. C. Cunha et al., “Controversies about the chromosomal stability of cultivated mesenchymal stem cells: their clinical use is it safe?” Current Stem Cell Research and Therapy, vol. 7, no. 5, pp. 356–363, 2012. View at Publisher · View at Google Scholar · View at Scopus
  18. A.-M. Rodriguez, C. Elabd, F. Delteil et al., “Adipocyte differentiation of multipotent cells established from human adipose tissue,” Biochemical and Biophysical Research Communications, vol. 315, no. 2, pp. 255–263, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. K. Gonda, T. Shigeura, T. Sato et al., “Preserved proliferative capacity and multipotency of human adipose-derived stem cells after long-term cryopreservation,” Plastic and Reconstructive Surgery, vol. 121, no. 2, pp. 401–410, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. K. Oishi, H. Noguchi, H. Yukawa et al., “Cryopreservation of mouse adipose tissue-derived stem/progenitor cells,” Cell Transplantation, vol. 17, no. 1-2, pp. 35–41, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. G. Minonzio, M. Corazza, L. Mariotta et al., “Frozen adipose-derived mesenchymal stem cells maintain high capability to grow and differentiate,” Cryobiology, vol. 69, no. 2, pp. 211–216, 2014. View at Publisher · View at Google Scholar · View at Scopus
  22. C. M. Kolf, E. Cho, and R. S. Tuan, “Mesenchymal stromal cells. Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation,” Arthritis Research & Therapy, vol. 9, no. 1, article 204, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. A. J. Katz, A. Tholpady, S. S. Tholpady, H. Shang, and R. C. Ogle, “Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells,” STEM CELLS, vol. 23, no. 3, pp. 412–423, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. L. Lei, W. Liao, P. Sheng, M. Fu, A. He, and G. Huang, “Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture,” Science in China, Series C: Life Sciences, vol. 50, no. 3, pp. 320–328, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. F. Djouad, B. Delorme, M. Maurice et al., “Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes,” Arthritis Research & Therapy, vol. 9, no. 2, article R33, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Mieno, R. T. Clements, M. Boodhwani et al., “Characteristics and function of cryopreserved bone marrow-derived endothelial progenitor cells,” Annals of Thoracic Surgery, vol. 85, no. 4, pp. 1361–1366, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. S. Thirumala, J. M. Gimble, and R. V. Devireddy, “Evaluation of methylcellulose and dimethyl sulfoxide as the cryoprotectants in a serum-free freezing media for cryopreservation of adipose-derived adult stem cells,” Stem Cells and Development, vol. 19, no. 4, pp. 513–522, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. M. L. González-Fernández, S. Pérez-Castrillo, P. Ordás-Fernández, M. E. López-González, B. Colaço, and V. Villar-Suárez, “Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cells for future use in regenerative medicine,” Cryobiology, vol. 71, no. 2, pp. 256–263, 2015. View at Publisher · View at Google Scholar