Research Article

Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers

Figure 3

IMR hiPS-cardiomyocytes (CMs) cultured on aligned fiber (AF)-coated and gelatin-coated (Flat) microelectrode arrays (MEA). (a) Phase contrast images of CMs plated on the MEA for 2 days: the scale bar in = 200 μm. (b, c) Fluorescence images of CMs cultured for 14 days on MEA. (b) MLC2v is shown in red, (c) β-MHC in green, and DAPI in blue. The scale bar = 200 μm. The color on fiber is due to autofluorescence of PMGI. White arrow marked the fiber orientation.