TY - JOUR A2 - Rangel, Maria C. AU - Catanzaro, Giuseppina AU - Besharat, Zein Mersini AU - Garg, Neha AU - Ronci, Maurizio AU - Pieroni, Luisa AU - Miele, Evelina AU - Mastronuzzi, Angela AU - Carai, Andrea AU - Alfano, Vincenzo AU - Po, Agnese AU - Screpanti, Isabella AU - Locatelli, Franco AU - Urbani, Andrea AU - Ferretti, Elisabetta PY - 2016 DA - 2016/01/06 TI - MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs SP - 2683042 VL - 2016 AB - Medulloblastoma (MB) is the most common malignant brain tumor of pediatric age and is characterized by cells expressing stem, astroglial, and neuronal markers. Among them, stem-like cells (hMB-SLCs) represent a fraction of the tumor cell population with the potential of self-renewal and proliferation and have been associated with tumor poor prognosis. In this context, microRNAs have been described as playing a pivotal role in stem cells differentiation. In our paper, we analyze microRNAs profile and genes expression of hMB-SLCs before and after Retinoic Acid- (RA-) induced differentiation. We aimed to identify pivotal players of specific pathways sustaining stemness and/or tumor development and progression and integrate the results of our recent proteomic study. Our results uncovered 22 differentially expressed microRNAs that were used as input together with deregulated genes and proteins in the Genomatix Pathway System (GePS) analysis revealing 3 subnetworks that could be interestingly involved in the maintenance of hMB-SLCs proliferation. Taken together, our findings highlight microRNAs, genes, and proteins that are significantly modulated in hMB-SLCs with respect to their RA-differentiated counterparts and could open new perspectives for prognostic and therapeutic intervention on MB. SN - 1687-966X UR - https://doi.org/10.1155/2016/2683042 DO - 10.1155/2016/2683042 JF - Stem Cells International PB - Hindawi Publishing Corporation KW - ER -