Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs

Figure 3

(a) Gene expression analysis of BAMBI and (b) BMP4 normalized to HPRT in MSC pellet cultures under chondrogenic and hypertrophy enhancing conditions analysed by real-time PCR. BAMBI and BMP4 are significantly upregulated under hypertrophic conditions compared to chondrogenic controls on days 17, 21, and 28. different donors, . (c) Western blot analysis of BAMBI: increased amount of BAMBI protein can be detected under hypertrophic conditions on days 21 and 28 compared to chondrogenic conditions.

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