Improvement in Isolation and Identification of Mouse Oogonial Stem Cells

Figure 2

Transfection of OSCs and transplantation into ovaries of recipient mice. (a) Schematic diagram for the lentivirus vector with EGFP. LTR, long terminal repeat; pUbi, ubiquitin promoter. (b) Immunofluorescence-based analysis (right) and bright field view (left) of OSCs transfected with EGFP vector. (c) The ovaries from the recipient were dissected 12 days after injection with EGFP-expressing OSCs and the oocytes within these ovaries were visualized under fluorescence microscope. EGFP-positive oocyte (above) displayed fluorescence and the negative one (below) showed no fluorescence. (d, e) The fetuses from the mated recipients were obtained and observed under fluorescence microscope under the same exposure time. (d) On the top is the overview of the EGFP-positive fetus under stereoscope and at the bottom are the images from the head, body, and tail section showing fluorescence. (e) On the top is the EGFP-negative fetus under stereoscope and at the bottom are the images showing no signal in three sections under fluorescence microscope.

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