Stem Cells International

Stem Cells International / 2016 / Article
Special Issue

Vascular Diseases and Metabolic Disorders

View this Special Issue

Research Article | Open Access

Volume 2016 |Article ID 2916017 | 8 pages | https://doi.org/10.1155/2016/2916017

Endothelium-Independent Hypoxic Contraction Is Prevented Specifically by Nitroglycerin via Inhibition of Akt Kinase in Porcine Coronary Artery

Academic Editor: Yingmei Feng
Received04 Aug 2015
Revised10 Oct 2015
Accepted13 Oct 2015
Published29 Dec 2015

Abstract

Objective. Hypoxia-induced sustained contraction of porcine coronary artery is endothelium-independent and mediated by PI3K/Akt/Rho kinase. Nitroglycerin (NTG) is a vasodilator used to treat angina pectoris and acute heart failure. The present study was to determine the role of NTG in hypoxia-induced endothelium-independent contraction and the underlying mechanism. Methods and Results. Organ chamber technique was used to measure the isometric vessel tension of isolated porcine coronary arteries. Protein levels of phosphorylated and total Akt were determined by western blot. A sustained contraction of porcine coronary arteries induced by hypoxia was significantly reduced by NTG but not by isoproterenol. This contraction was also inhibited by DETA NONOate, 8-Br-cGMP, which can be reversed by ODQ, and Rp-8-Br-PET-cGMPS. The restored contraction was blocked by LY294002. The reduction of Akt-p at Ser-473 by NTG, DETA NONOate, and 8-Br-cGMP was significantly inhibited by ODQ, PKG-I. The decrease in Akt-p level by NTG and 8-Br-cGMP was prevented by calyculin A but not by okadaic acid. Conclusions. These results demonstrated that the endothelium-independent sustained hypoxic vasoconstriction can be prevented by NTG and that the inhibition of PI3K/Akt signaling pathway may be involved.

1. Introduction

Since it was firstly reported by Vanhoutte in 1976 that acute hypoxia caused a rapid increase in tension of canine saphenous veins precontracted with acetylcholine [1], hypoxic vasoconstriction has been observed in a number of systemic vessel types including coronary arteries [29]. Subsequent studies demonstrate that the rapid hypoxic contraction is endothelium-dependent and closely associated with nitric oxide (NO) and soluble guanylyl cyclase (sGC) but unrelated to cGMP [4, 7, 8]. Our recent study revealed that cIMP, which was formed by sGC under hypoxic conditions, may play a role in the hypoxia-induced vasoconstriction [9]. This rapid endothelium-dependent hypoxic vasoconstriction is usually followed by a transient relaxation and then a sustained contraction in porcine coronary arteries when exposed to hypoxia. Our recent study suggests that this sustained hypoxic contraction is endothelium-independent and mediated by PI3K/Akt/Rho kinase [10].

Akt is a serine/threonine kinase which plays important roles in cell survival, differentiation, proliferation, metabolism, migration, and apoptosis [1116]. It is increasingly recognized as being a pivotal modulator of vascular tone by regulating endothelial nitric oxide synthase, L-type calcium channel, Rho kinase, and phosphodiesterase type 5 [1725]. Activation of Akt depends on the integrity of its pleckstrin homology (PH) domain, which is required for the membrane translocation of the kinase. After being translocated to the membrane via binding of phosphatidylinositol (3,4,5)-triphosphates, Akt is phosphorylated by its upstream kinases at Thr-308 and then at Ser-473 [26]. Akt is not fully activated until both Thr-308 and Ser-473 are phosphorylated. Therefore, the full activation of Akt depends on the phosphorylation of Ser-473 [27]. Our recent study showed that the hypoxia-induced Akt phosphorylation at Ser-473 is associated with changes in the tension of porcine coronary arteries [10].

Nitroglycerin (NTG) is a commonly used medicine in the treatment of angina pectoris and acute heart failure. It causes vasodilatation after being converted into NO or a NO related intermediate in the cytoplasm, followed by cGMP elevation due to activation of sGC and PKG [28, 29]. Our recent study showed that Akt activity was inhibited by NO via inhibiting the phosphorylation at Ser-473 of the enzyme in porcine pulmonary artery [30]. The present study was intended to determine whether the endothelium-independent hypoxic contraction is prevented by NTG via inhibition of Akt in porcine coronary artery.

2. Materials and Methods

2.1. Reagents

The following drugs were used (unless otherwise specified, all were obtained from Sigma, St. Louis, MO, USA): 8-Br-cGMP (8-bromo-guanosine 3′5′-cyclic monophosphate), calyculin A, DETA NONOate[2,2′-(hydroxynitrosohydrazono) bis(ethanamine)], LY294002 [2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride], nitroglycerin (NTG, Beijing Yimin Pharmaceutical Co., Ltd., Beijing, China), okadaic acid, Rp isomer (Rp-8-Br-PET-cGMPS; Biolog Life Science Institute, Bremen, Germany), and U46619 (9,11-Dideoxy-11α,9α-epoxymethanoprostaglandin F2α).

LY294002, calyculin A, and okadaic acid were dissolved in DMSO (final concentration; <0.2%). Preliminary experiments showed that DMSO at the concentration used had no effect on contraction to U46619 and relaxation induced by nitroglycerin. The other drugs were prepared using distilled water.

2.2. Porcine Coronary Arteries Preparations

Fresh porcine hearts were collected from a local slaughterhouse. The left anterior descending coronary arteries were carefully dissected and cut into rings in ice-cold modified Krebs-Ringer bicarbonate buffer. The vessels were denuded of the endothelium mechanically by inserting the tips of a watchmaker’s forceps into the lumen of the vessel and rolling the vessel back and forth on saline-saturated filter paper. Animal handling and study protocols were in accordance with US National Institutes of Health guidelines. They were reviewed and approved by Animal Care and Use Review Committees of Peking University Health Science Center [31].

2.3. Vessel Tension Study

Rings of porcine coronary arteries were repeatedly rinsed and suspended in organ chambers filled with 10 mL of the modified Krebs-Ringer bicarbonate solution maintained at °C and aerated with 95% O2-5% CO2 (pH = 7.4). Two stirrups passed through the lumen of the vessel ring: one was anchored to the bottom of the organ chamber and the other was connected to a strain gauge. The isometric force was measured with ML785 PowerLab/8sp recording and Analysis System (ADInstruments Pty Ltd., Castle Hill, Australia) [32].

At the beginning of the experiment, each vessel was stretched to its optimal resting tension by stepwise stretching until the contraction of the vessel ring to 100 mM KCl reached a plateau. The optimal resting tension of porcine coronary arteries was ~2.5 g. Then one hour of equilibration was allowed. After equilibration certain inhibitors including ODQ, Rp-8-Br-PET-cGMPS, and LY294002 were added and remained throughout the experiment. In some experiments, NTG, isoproterenol, DETA NONOate, and/or 8-Br-cGMP were added 30 minutes before constriction. The effects of hypoxia (95% N2-5% CO2) were examined in vessels preconstricted with U46619 to a similar tension level. To eliminate the possible involvement of endogenous prostanoids and endothelium-derived nitric oxide, indomethacin (10−5 M, an inhibitor of cyclooxygenase) and nitro-L-arginine (10−4 M, an inhibitor of NO synthase) were administrated. All experiments were carried out in a parallel fashion.

2.4. Western Blot Study

Pretreatment of arterial rings: isolated porcine coronary arteries without endothelium were incubated in Krebs-Ringer bicarbonate buffer maintained at °C and aerated with 95% O2-5% CO2 (pH = 7.4) in the presence of solvent, NTG, DETA NONOate, or 8-Br-cGMP. The inhibitors including ODQ, Rp-Br-PET-cGMPS, calyculin A, and okadaic acid were added at least 30 minutes before testing their effects.

Immunodetection: vessel rings were rapidly frozen with liquid nitrogen and homogenized in lysis buffer containing 34.67 mM SDS, 1 mM sodium orthovanadate, and 10 mM Tris base (pH 7.4). Tissue lysates each containing 20 μg of soluble protein were subjected to SDS-PAGE and electrotransferred to polyvinylidene fluoride membrane. Nonspecific binding of antibody was blocked by washing with TBS buffer containing 5% milk for 1 hour at room temperature. After two times brief washing with TBS plus 0.1% Tween-20 (TBS-T), the blot was incubated with the first antibody of Akt (Cell Signaling Technology, MA, USA; 1 : 1000 dilution) or Akt-p (S473) (Cell Signaling Technology, MA, USA; 1 : 1000 dilution) overnight at 4°C. Afterwards, the blot was washed three times with TBS-T buffer and then incubated with the secondary antibody for 1 hour at room temperature followed by another 3 times of washing and then developed using the chemiluminescent detection method (Amersham ECL). Proteins of Akt or Akt-p were quantified by densitometry using a Gel Doc 2000 densitometer (BIO-RAD, CA, USA) and normalized to scanning signals of beta-actin (Calbiochem, CA, USA).

2.5. Data Analysis

Data are shown as means ± SEM. Student’s -test for unpaired observations was used to compare the mean values of two groups. Mean values of more than two groups were compared using one-way ANOVA test, with the Student-Newman-Keuls test for post hoc testing of multiple comparisons. Statistical significance was accepted when the value (two tailed) was less than 0.05. In all experiments, represents the number of animals.

3. Results

3.1. Hypoxia-Induced Sustained Vasoconstriction Was Prevented Specifically by NTG

Rings of porcine coronary arteries without endothelium were preconstricted with U46619 (3 × 10−7 M, a thromboxane A2 analog) before testing the response of hypoxia (95% N2-5% CO2). Hypoxia induced a rapid relaxation in the first 30 min, which was followed by a sustained contraction in porcine coronary arteries (Figure 1(a)). This sustained contraction of porcine coronary arteries was prevented by NTG (10−6 M; Figure 1(a)), but not by isoproterenol (ISO, 10−7 M; Figure 1(a)). Our preliminary experiment showed that isoproterenol (10−7 M) induced the same relaxation as NTG (10−6 M) in porcine coronary arteries preconstricted with U46619 (3 × 10−7 M, data not shown).

3.2. Involvement of NO/sGC/cGMP/PKG Pathway

The prevention of the hypoxic-induced vasoconstriction caused by NTG (10−6 M) could be recovered by ODQ (3 × 10−5 M), a specific inhibitor of sGC (Figure 1(b)). Pretreatment of coronary arteries with NTG downstream molecule, NO donor (DETA NONOate, 10−5 M), or cGMP analog (8-Br-cGMP, 10−4 M) also attenuated the vasoconstriction caused by continuous hypoxia. These effects were blocked by ODQ or the inhibitor of PKG (PKG-I, Rp-8-Br-PET-cGMPS, 3 × 10−5 M). ODQ and PKG-I themselves had no effect on the hypoxic vasoconstriction (Figures 1(b), 1(c) and 1(d)).

3.3. Role of PI3K/Akt

The prevention of hypoxia-induced vasoconstriction of porcine coronary arteries caused by NTG, NO, or cGMP could be recovered by ODQ or PKG-I and the restored contractions were largely abolished by coincubation with LY294002, a specific inhibitor of PI3K (Figures 1(b), 1(c) and 1(d)).

3.4. Involvement of sGC in the Downregulation of Akt-p (S473) by NTG and NO

The protein levels of phosphorylated Akt at Ser-473 were significantly reduced by incubation with NTG (10−5 M) for 15 and 30 minutes (Figure 2(a)) or by incubation with DETA NONOate (10−4 M) for 1 to 30 minutes (Figure 2(c)). These effects were prevented by ODQ (3 × 10−5 M), a specific inhibitor of sGC (Figures 2(b) and 2(d)).

3.5. Involvement of PKG in the Downregulation of Akt-p (S473) by 8-Br-cGMP

The protein levels of phosphorylated Akt at Ser-473 were also significantly reduced by incubation with 8-Br-cGMP (10−4 M) for 45 to 90 minutes. The effect was blocked by Rp-8-Br-PET-cGMPS (3 × 10−5 M), a specific inhibitor of PKG (Figure 3).

3.6. Involvement of PP1 in the Downregulation of Akt-p (S473) by NTG and 8-Br-cGMP

Akt-p/Akt was decreased when treated with NTG (10−6 M; Figure 4(a)) for 15 min and 8-Br-cGMP (cGMP analog, 10−4 M; Figure 4(b)) for 60 min. Calyculin A (10−7 M, an inhibitor of PP1 and PP2A) but not okadaic acid (10−7 M, an inhibitor of PP2A) prevented the decrease in Akt-p (S473) caused by NTG and 8-Br-cGMP (Figure 4).

4. Discussion

Coronary artery spasm is a risk factor of acute ischemia heart disease such as angina pectoris and acute coronary syndrome [33, 34]. Endothelial dysfunction, hyperactivity of vascular smooth muscle cells, and other factors, including hypoxia, may be involved in the development of coronary vasospasm [2, 3, 34]. Recently, we found that prolonged hypoxia induced a transient initial contraction followed by a short term relaxation and a sustained contraction in porcine coronary arteries [10]. The first rapid hypoxic contraction is endothelium- or NO-dependent as reported by Vanhoutte and others [4, 9] while the second sustained contraction triggered by hypoxia is endothelium-independent [10]. Our study suggests that when oxygen content in the blood is decreased under certain disease conditions such as sleep apnea, high altitude sickness, and chronic obstructive pulmonary disease, the sustained hypoxic vasoconstriction may contribute to the development of spasm in coronary artery.

Both NTG and isoproterenol are vasodilators used in the treatment of cardiovascular disease. It is well known that vasodilatation caused by NTG is predominantly mediated by sGC/cGMP signaling pathway [28, 29] and the relaxation caused by isoproterenol involves adenylyl cyclase/cAMP signaling [35]. In our study, preincubation of nitroglycerin prevented the sustained vasoconstriction induced by hypoxia. By contrast, treatment of coronary arteries with isoproterenol had little effect. These results suggest that the endothelium-independent hypoxia-induced contraction is prevented specifically by NTG.

NTG causes vasodilatation via the intracellular conversion to NO or a NO related intermediate, which elevates cGMP by activating sGC [28, 29]. We found that the endothelium-independent hypoxic contraction was also prevented by NO. The suppression of hypoxic vasoconstriction by NTG or NO was recovered by ODQ, a specific inhibitor of sGC, suggesting that cGMP is involved in the effect of NTG and NO on hypoxia-induced vasoconstriction. PKG is one of the primary targets of cGMP. In our study the cGMP analog decreased the contraction induced by prolonged hypoxia in a manner sensitive to the inhibition of PKG by Rp-8-Br-PET-cGMPS. Thus, the activation of NO/sGC/cGMP/PKG pathway is involved.

Our previous work demonstrates that the activation of PI3K/Akt by hypoxia plays an important role in hypoxia-induced vasoconstriction [10]. This hypoxia-induced Akt phosphorylation at Ser-473 and vasoconstriction could be abolished by LY294002, an inhibitor of PI3K [10]. The present study shows that inhibition of PI3K/Akt pathway by LY294002 inhibited the effect of ODQ and Rp-8-Br-PET-cGMPS on the suppression of hypoxic vasoconstriction caused by NTG, NO, and 8-Br-cGMP. Hence, it appears that NTG prevents the hypoxia-induced coronary vasoconstriction mainly by inhibition of PI3K/Akt pathway.

Akt is a serine/threonine protein kinase involved in various cellular processes including the modulation of vascular smooth muscle responses [17]. In the present study the treatments of porcine coronary arteries with NTG, NO, or 8-Br-cGMP reduced the Akt phosphorylation at Ser-473, which was blocked by the inhibitors of sGC and PKG. It suggests that PKG activated by cGMP exerts an inhibitory effect on Akt by decreasing the phosphorylation of Akt at Ser-473, which is in line with our observation obtained in porcine pulmonary arteries [30].

PKG exerts its effects by phosphorylating the target proteins, including calcium activated potassium channels (BK channels) [36], IP3R-associated PKG substrate [37], RhoA [38], and myosin phosphatase targeting subunit (MYPT1) [39]. The present study showed that Akt was dephosphorylated rather than phosphorylated by PKG, indicating that PKG might act on Akt indirectly. Indeed, some studies show that dephosphorylation of Akt is mediated by protein phosphatase 1 (PP1) or protein phosphatase 2A (PP2A) [40]. To determine whether PP1 and PP2A played a role in the dephosphorylation of Akt caused by PKG, the effects of calyculin A (CA), a nonselective inhibitor of PP1 and PP2A, and okadaic acid (OA), a selective inhibitor of PP2A, were studied [41, 42]. We found that the reduced phosphorylation of Akt induced by NTG and cGMP was reversed by CA but not by OA. Therefore, PKG may reduce the phosphorylation of Akt at Ser-473 mostly through PP1.

We herein demonstrate that pretreatment of NTG can prevent endothelium-independent vasoconstriction caused by prolonged hypoxia in porcine coronary arteries. The underlying mechanism may involve the activation of PKG induced by NTG, NO, or cGMP, which subsequently attenuates the phosphorylation of Akt at Ser-473 through PP1 in porcine coronary arteries, resulting in the inhibition of PI3K/Akt signaling pathway.

Conflict of Interests

All of the authors have no conflict of interests.

Authors’ Contribution

Huixia Liu, Yanjing Li, and Yuanming An contributed equally to the research presented in this paper.

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant no. 81373404), Doctoral Fund of Ministry of Education for New Teachers (Grant no. 20100001120037), and Beijing Higher Education Young Elite Teacher Project (Grant no. YETP0054).

References

  1. P. M. Vanhoutte, “Effects of anoxia and glucose depletion on isolated veins of the dog,” American Journal of Physiology, vol. 230, no. 5, pp. 1261–1268, 1976. View at: Google Scholar
  2. M. Karmazyn, R. E. Beamish, and N. S. Dhalla, “Involvement of calcium in coronary vasoconstriction due to prolonged hypoxia,” American Heart Journal, vol. 107, no. 2, pp. 293–297, 1984. View at: Publisher Site | Google Scholar
  3. J. M. Van Nuete, J. Van Beek, and P. M. Vanhoutte, “Inhibitory effect of lidoflazine on contractions of isolated canine coronary arteries caused by norepinephrine, 5-hydroxytryptamine, high potassium, anoxia and ergonovine maleate,” Journal of Pharmacology and Experimental Therapeutics, vol. 213, no. 1, pp. 179–187, 1980. View at: Google Scholar
  4. C. K. Y. Chan, J. Mak, Y. Gao, R. Y. K. Man, and P. M. Vanhoutte, “Endothelium-derived NO, but not cyclic GMP, is required for hypoxic augmentation in isolated porcine coronary arteries,” American Journal of Physiology—Heart and Circulatory Physiology, vol. 301, no. 6, pp. H2313–H2321, 2011. View at: Publisher Site | Google Scholar
  5. J. G. De Mey and P. M. Vanhoutte, “Contribution of the endothelium to the response to anoxia in the canine femoral artery,” Archives Internationales de Pharmacodynamie et de Therapie, vol. 253, no. 2, pp. 325–326, 1981. View at: Google Scholar
  6. S. Dhein, A. Salameh, and W. Klaus, “A new endothelium-dependent vasoconstricting factor (EDCF) in pig coronary artery,” European Heart Journal, vol. 10, pp. 82–85, 1989. View at: Google Scholar
  7. T. Graser and P. M. Vanhoutte, “Hypoxic contraction of canine coronary arteries: role of endothelium and cGMP,” The American Journal of Physiology—Heart and Circulatory Physiology, vol. 261, no. 6, pp. H1769–H1777, 1991. View at: Google Scholar
  8. P. J. Pearson, P. J. Lin, H. V. Schaff, and P. M. Vanhoutte, “Augmented endothelium-dependent constriction to hypoxia early and late following reperfusion of the canine coronary artery,” Clinical and Experimental Pharmacology and Physiology, vol. 23, no. 8, pp. 634–641, 1996. View at: Publisher Site | Google Scholar
  9. Z. Chen, X. Zhang, L. Ying et al., “cIMP synthesized by sGC as a mediator of hypoxic contraction of coronary arteries,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 307, no. 3, pp. H328–H336, 2014. View at: Publisher Site | Google Scholar
  10. H. Liu, Z. Chen, J. Liu, L. Liu, Y. Gao, and D. Dou, “Endothelium-independent hypoxic contraction of porcine coronary arteries may be mediated by activation of phosphoinositide 3-kinase/Akt pathway,” Vascular Pharmacology, vol. 61, no. 2-3, pp. 56–62, 2014. View at: Publisher Site | Google Scholar
  11. V. Duronio, “The life of a cell: apoptosis regulation by the PI3K/PKB pathway,” Biochemical Journal, vol. 415, no. 3, pp. 333–344, 2008. View at: Publisher Site | Google Scholar
  12. L. C. Foukas, I. M. Berenjeno, A. Gray, A. Khwaja, and B. Vanhaesebroeck, “Activity of any class IA PI3K isoform can sustain cell proliferation and survival,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 25, pp. 11381–11386, 2010. View at: Publisher Site | Google Scholar
  13. A. Fujino, N. A. Arango, Y. Zhan et al., “Cell migration and activated PI3K/AKT-directed elongation in the developing rat Müllerian duct,” Developmental Biology, vol. 325, no. 2, pp. 351–362, 2009. View at: Publisher Site | Google Scholar
  14. Y. Xu, J. W. Hill, M. Fukuda et al., “PI3K signaling in the ventromedial hypothalamic nucleus is required for normal energy homeostasis,” Cell Metabolism, vol. 12, no. 1, pp. 88–95, 2010. View at: Publisher Site | Google Scholar
  15. L. Zhang, D. Xing, X. Gao, and S. Wu, “Low-power laser irradiation promotes cell proliferation by activating PI3K/Akt pathway,” Journal of Cellular Physiology, vol. 219, no. 3, pp. 553–562, 2009. View at: Publisher Site | Google Scholar
  16. S. Martínez-Herrero, I. M. Larráyoz, L. Ochoa-Callejero, J. García-Sanmartín, and A. Martínez, “Adrenomedullin as a growth and cell fate regulatory factor for adult neural stem cells,” Stem Cells International, vol. 2012, Article ID 804717, 18 pages, 2012. View at: Publisher Site | Google Scholar
  17. M. Sata and R. Nagai, “Phosphatidylinositol 3-kinase: a key regulator of vascular tone?” Circulation Research, vol. 91, no. 4, pp. 273–275, 2002. View at: Publisher Site | Google Scholar
  18. C. Le Blanc, C. Mironneau, C. Barbot et al., “Regulation of vascular L-type Ca2+ channels by phosphatidylinositol 3,4,5-trisphosphate,” Circulation Research, vol. 95, no. 3, pp. 300–307, 2004. View at: Publisher Site | Google Scholar
  19. N. Macrez, C. Mironneau, V. Carricaburu et al., “Phosphoinositide 3-kinase isoforms selectively couple receptors to vascular L-type Ca2+ channels,” Circulation Research, vol. 89, no. 8, pp. 692–699, 2001. View at: Publisher Site | Google Scholar
  20. J.-F. Quignard, J. Mironneau, V. Carricaburu et al., “Phosphoinositide 3-kinase γ mediates angiotensin II-induced stimulation of L-type calcium channels in vascular myocytes,” The Journal of Biological Chemistry, vol. 276, no. 35, pp. 32545–32551, 2001. View at: Publisher Site | Google Scholar
  21. P. Viard, T. Exner, U. Maier, J. Mironneau, B. Nürnberg, and N. Macrez, “Gβγ dimers stimulate vascular L-type Ca2+ channels via phosphoinositide 3-kinase,” The FASEB Journal, vol. 13, no. 6, pp. 685–694, 1999. View at: Google Scholar
  22. P. Viard, N. Macrez, C. Mironneau, and J. Mironneau, “Involvement of both G protein alphas and beta gamma subunits in beta-adrenergic stimulation of vascular L-type Ca2+ channels,” British Journal of Pharmacology, vol. 132, no. 3, pp. 669–676, 2001. View at: Publisher Site | Google Scholar
  23. L. Miao, Y. Dai, and J. Zhang, “Mechanism of RhoA/Rho kinase activation in endothelin-1-induced contraction in rabbit basilar artery,” American Journal of Physiology: Heart and Circulatory Physiology, vol. 283, no. 3, pp. H983–H989, 2002. View at: Publisher Site | Google Scholar
  24. Y. M. Seok, M. A. Azam, Y. Okamoto et al., “Enhanced Ca2+-dependent activation of phosphoinositide 3-kinase class IIα isoform-Rho axis in blood vessels of spontaneously hypertensive rats,” Hypertension, vol. 56, no. 5, pp. 934–941, 2010. View at: Publisher Site | Google Scholar
  25. E. A. Wehrwein, C. A. Northcott, R. D. Loberg, and S. W. Watts, “Rho/Rho kinase and phosphoinositide 3-kinase are parallel pathways in the development of spontaneous arterial tone in deoxycorticosterone acetate-salt hypertension,” Journal of Pharmacology and Experimental Therapeutics, vol. 309, no. 3, pp. 1011–1019, 2004. View at: Publisher Site | Google Scholar
  26. T. O. Chan, S. E. Rittenhouse, and P. N. Tsichlis, “AKT/PKB and other D3 phosphoinositide-regulated kinases: kinase activation by phosphoinositide-dependent phosphorylation,” Annual Review of Biochemistry, vol. 68, pp. 965–1014, 1999. View at: Publisher Site | Google Scholar
  27. D. R. Alessi, M. Andjelkovic, B. Caudwell et al., “Mechanism of activation of protein kinase B by insulin and IGF-1,” The EMBO Journal, vol. 15, no. 23, pp. 6541–6551, 1996. View at: Google Scholar
  28. X. Qin, X. Zheng, H. Qi, D. Dou, J. U. Raj, and Y. Gao, “cGMP-dependent protein kinase in regulation of basal tone and in nitroglycerin- and nitric-oxide-induced relaxation in porcine coronary artery,” Pflügers Archiv European Journal of Physiology, vol. 454, no. 6, pp. 913–923, 2007. View at: Publisher Site | Google Scholar
  29. H. Qi, X. Zheng, X. Qin et al., “Protein kinase G regulates the basal tension and plays a major role in nitrovasodilator-induced relaxation of porcine coronary veins,” British Journal of Pharmacology, vol. 152, no. 7, pp. 1060–1069, 2007. View at: Publisher Site | Google Scholar
  30. J. Liu, H. Liu, Y. Li et al., “Cross regulation between CGMP-dependent protein kinase and AKT in vasodilatation of porcine pulmonary artery,” Journal of Cardiovascular Pharmacology, vol. 64, no. 5, pp. 452–459, 2014. View at: Publisher Site | Google Scholar
  31. D. Dou, X. Zheng, X. Qin et al., “Role of cGMP-dependent protein kinase in development of tolerance to nitroglycerine in porcine coronary arteries,” British Journal of Pharmacology, vol. 153, no. 3, pp. 497–507, 2008. View at: Publisher Site | Google Scholar
  32. D. Dou, H. Ma, X. Zheng et al., “Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance,” Cardiovascular Research, vol. 86, no. 1, pp. 151–159, 2010. View at: Publisher Site | Google Scholar
  33. H. Kawano and K. Node, “The role of vascular failure in coronary artery spasm,” Journal of Cardiology, vol. 57, no. 1, pp. 2–7, 2011. View at: Publisher Site | Google Scholar
  34. G. A. Lanza, G. Careri, and F. Crea, “Mechanisms of coronary artery spasm,” Circulation, vol. 124, no. 16, pp. 1774–1782, 2011. View at: Publisher Site | Google Scholar
  35. N. Flacco, V. Segura, M. Perez-Aso et al., “Different β-adrenoceptor subtypes coupling to cAMP or NO/cGMP pathways: implications in the relaxant response of rat conductance and resistance vessels,” British Journal of Pharmacology, vol. 169, no. 2, pp. 413–425, 2013. View at: Publisher Site | Google Scholar
  36. M. Fukao, H. S. Mason, F. C. Britton, J. L. Kenyon, B. Horowitz, and K. D. Keef, “Cyclic GMP-dependent protein kinase activates cloned BKCa channels expressed in mammalian cells by direct phosphorylation at serine 1072,” The Journal of Biological Chemistry, vol. 274, no. 16, pp. 10927–10935, 1999. View at: Publisher Site | Google Scholar
  37. A. Geiselhöringer, M. Werner, K. Sigl et al., “IRAG is essential for relaxation of receptor-triggered smooth muscle contraction by cGMP kinase,” The EMBO Journal, vol. 23, no. 21, pp. 4222–4231, 2004. View at: Publisher Site | Google Scholar
  38. G. Loirand, C. Guilluy, and P. Pacaud, “Regulation of Rho proteins by phosphorylation in the cardiovascular system,” Trends in Cardiovascular Medicine, vol. 16, no. 6, pp. 199–204, 2006. View at: Publisher Site | Google Scholar
  39. Y. Gao, A. D. Portugal, S. Negash, W. Zhou, L. D. Longo, and J. U. Raj, “Role of Rho kinases in PKG-mediated relaxation of pulmonary arteries of fetal lambs exposed to chronic high altitude hypoxia,” American Journal of Physiology—Lung Cellular and Molecular Physiology, vol. 292, no. 3, pp. L678–L684, 2007. View at: Publisher Site | Google Scholar
  40. L. Li, C. H. Ren, S. A. Tahir, C. Ren, and T. C. Thompson, “Caveolin-1 maintains activated Akt in prostate cancer cells through scaffolding domain binding site interactions with and inhibition of serine/threonine protein phosphatases PP1 and PP2A,” Molecular and Cellular Biology, vol. 23, no. 24, pp. 9389–9404, 2003. View at: Publisher Site | Google Scholar
  41. B. Favre, P. Turowski, and B. A. Hemmings, “Differential inhibition and posttranslational modification of protein phosphatase 1 and 2A in MCF7 cells treated with calyculin-A, okadaic acid, and tautomycin,” The Journal of Biological Chemistry, vol. 272, no. 21, pp. 13856–13863, 1997. View at: Publisher Site | Google Scholar
  42. H. Ishihara, B. L. Martin, D. L. Brautigan et al., “Calyculin A and okadaic acid: Inhibitors of protein phosphatase activity,” Biochemical and Biophysical Research Communications, vol. 159, no. 3, pp. 871–877, 1989. View at: Publisher Site | Google Scholar

Copyright © 2016 Huixia Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

682 Views | 368 Downloads | 3 Citations
 PDF  Download Citation  Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.